Systems provide more control than manual purification because of the ability to automatically control the flow rate and monitor the progress of the purification as well as to make controlled gradients and automatically collect fractions. M925 mixing chamber, inv907 valve, p960 pump, air900 air monitor. For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin. Data file 28915986 ad readytoprocess akta ready system fig 1. Aktaprime plus user manual 11002644 edition aa 9 1introduction this section is an introduction to aktaprime plus, the documentation included and the content in this manual. Complete amersham aktaexplorer 100 system from 72006. Akta explorer systems include an extensive column library with details of run parameters for each prepacked column.
The method magician and optimized system configurations enable rapid networking viewing, method scouting, procedure optimization, and. The method magician and optimized system configurations enable rapid networking viewing, method scouting, procedure optimization, and also scaleup experiments. Our hplc systems such as the agilent 1100 and 1200 series, akta fplcs, and. Ge healthcare amersham pharmacia akta explorer 100 fplc complete system ble16001248. Akta xpress user manual is a full service general contracting and construction management firm providing services throughout north carolina raleigh, including renovation construction, construction.
If the volume of rinsing solution in the storage bottle has decreased significantly, check if the rinsing system connectors are mounted properly. Akta explorer 10 or 100 fplc system, complete with 90day. Monitor uv900 user manual 18112005 edition af 7 1 introduction 1. Laboratory controls offers a best in the business full 90 day warranty on all refurbished items. The proven design has been verified during development and can be userconfigured to meet specific process demands. Check the operation of the pump according to the pump p900 user manual. Amersham biosciences akta prime fplc system laboratory. Fplc pharmacia akta explorer 10s 2002, fplc pharmacia akta explorer 10s 2002. Akta ready system 1 inlet manifold, 2 pump tubing, and 3 main flow path. The method wizard and optimized system configurations, allow rapid media screening, method scouting, method optimization, and scaleup experiments. Opening the unicorn software windows for manual control and programmable features purgingpriming pumps and tubing. Amersham biosciences ge healthcare akta fplc system.
Akta xpress user manual is a full service general contracting and construction management firm providing services throughout north carolina raleigh, including renovation construction, construction engineering, construction project management, general contracting, new construction and commercial construction services. This warranty covers your part against any material and or craftsmanship issues within one full year from. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse. Technical details are found in the akta pure user manual and akta avant user manual. Manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1. Ge akta explorer certified fplc system gmi trusted lab. Fraction collector frac950 user manual 181956 edition ae 7 1 introduction 1. It allows users to speed up process scaleup and cleaning between product. Aktaexplorer 100 cytiva, formerly ge healthcare life. Sections 1 5 contain the necessary information for operating the instrument. Apr 01, 2015 issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. It also includes relevant guidance for practical handling and maintenance of instrument components.
The akta fplc presents a complete integrated fplc system used for laboratoryscale purification of proteins and peptides. Ge akta explorer amersham pharmacia certified fplc system. Use of this web site constitutes acceptance of the labx user agreement. Ge amersham akta explorer fplc liquid chromatography. Check the valves according to instructions in the valve inv907 instructions valve ivpv908 instructions valve sv903 instructions. Flexible and intuitive chromatography system to meet your purification challenges in research applications. This high flow rate is necessary when working with.
Store the installation guide in the user manual box. Akta pure cytiva, formerly ge healthcare life sciences. We specialize in the full suite of akta fplc systems, molecular devices spectramax microplate readers, biotek microplate washers, ymc formerly lewa process technologies systems, chromacon contichrom instruments, and other niche laboratory equipment lines. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Akta ready is a chromatography system using a disposable flow path. This automatic configuration protects the system from damaging events including over pressure. In load mode, the sle loop is connected to the injection port, which cannot. Ge healthcare amersham pharmacia akta explorer 100 fplc.
Some fast protein liquid chromatography systems also have column switching valves that allow the user to switch between columns, sample pumps to apply the sample to a. Standard description notes harmonizedwith 2004108ec emcemissionsandimmunityrequirementsforelectricalequipmentfor measurement,controlandlaboratory use. They prefer to invest their idle time to talk or hang out. Fast protein liquid chromatography systems generally consist of a pump, a uv detector, a conductivity meter, and a fraction collector and operate at pressures of 3,500 psi 24 mpa. Some of our leading manufacturers include agilent, hp, waters, ge, amersham, dionex and shimazdzu. Contents monitor upc900 user manual 18112555 edition ag vii. All users must read this entire manual to fully understand the safe in which case the user might be required to take akta system has been established. Documents document view page ge pharmacia biotech akta. Tubing kit changes aktapurifier 100 high flow at delivery 0. The system will be operating on a software called unicorn 5. With flow rates up to 25 mlmin, akta avant 25 system is designed. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c ie n c a m e r s h a m b io s c ie box n c e monitor conductivity ph uv detector 3 wavelengths pumps column valves injector sample valve buffers phcell electrode sample holder pump a buffer switching valve outlet valve. Akta fplc explorer 100 operators manual marshall scientific. Akta explorer fplc system amersham pharmacia components with ge frac 950.
Akta avant is a preparative chromatography system intended for method and process development fig 1. Ge pharmacia akta purifier 100 gmi trusted laboratory. We offer an assortment of refurbished and used liquid chromatography solutions ranging from hplc, lcms to fplc. Unicorn is manual 18114045 important user information trademarks akta, fplc. Ensure your akta system is operating at peakperformance by priming the system and. Our systems can be customized to include almost any configuration of autosamplers, detectors and pumps. Amersham biosciences ge fplc system cambridge scientific. Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Replace the piston seals according to the user manual. Ge healthcare akta fplc frac950 fraction collector frac950 10593 duration. In this chapter this chapter contains the following sections. Check that injection valve is set to load column position. All users must read this entire manual to fully understand the safe use of akta. Aktaexplorer 100 cytiva ge healthcare life sciences.
Akta pure user manual, printed copy digital included. Akta explorer 10 or 100 fplc system, complete with 90day warranty. This handbook addresses different aspects of akta chromatography systems, such as the effect of system. Akta pure is a flexible and intuitive chromatography system. Akta fplc explorer 100 manual marshall scientific by marshall. When the user selects a column, the akta explorer instinctively sets the column volume, flow rate and pressure limit parameters into the method template.
Monitor upc900 user manual 18112555 edition ag 3 2 installation u p c 9 0. Ge akta explorer amersham pharmacia certified fplc system gmi. Akta explorer aktaexplorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples. I looked over the user manual for the device and the columns. All users must read this entire manual to fully understand the safe use of akta monitor upc900 workstation. Pdf pdf 163,93 kb 20031226 159e269c part 1 bst aj angelina jolie very sexy playboy pic. Up to 3 wavelengths can be monitored simultaneously.
View and download ge akta start maintenance manual online. Check valve is clogged or damaged, causing leakage. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m. Calibrate the electrode according to instructions monitor phc900 user manual.
Please see the aktadesign optimal configuration user manual for the recommended flow rates. If the leakage persists, replace the assembly according to instructions in the pump p960 user manual. It is intended to be operated as an integrated part of an aktadesign chromatography system running unicorn software. Box900, phc900 monitor, uv900 monitor, p900 pump, cu950 interface. Aktadesign purification user manual 8 2423 edition ad introduction to this handbook and unicorn 7. Akta fplc system operators manual marshall scientific. Amersham biosciences ge healthcare akta explorer 100 inquire about availability includes manuals, software, cu950, frac950, p960, iv908, inv907, air900, m925. Akta pure 25 new owners intro the exercise below will give a quick demonstration of how easy and intuitive the akta pure 25 will be for you in demonstrating downstream processing to your students. Fraction collector frac950 boyce thompson institute.
The small diameter, low flow kit manages flows of up to 175 lh using columns typically less than 20 cm. Aktaexplorer 100 air cytiva formerly ge healthcare life. Variable wavelength for detection in the range 190 700 nm. Jan 25, 2019 ge healthcare amersham pharmacia akta explorer 100 fplc complete system ble16001248. Available in two versions, akta avant systems have different flow ratepressure specifications but share the same hardware setup. Function the fraction collector collects fractions from akta pure purification runs. Atg provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. Amersham biosciences akta prime fplc system manual warranty information laboratory controls offers a best in the business full 90 day warranty on all refurbished items. Ge amersham akta explorer 100 fplc system asa global medical.
Amersham biosciences ge healthcare akta explorer 100. In the unicorn software, prompt the system control window. Ge amersham akta explorer 100 fplc chromatography systems are made for fast development and optimization of almost any biomolecular purification, utilizing one working platform for all samples and techniques. It also contains an overview of the safety instructions. The akta purifier 100 is perfect for application spanning a wide range of wavelengths the akta purifier 100 allows for rate flows of up to 100 ml per minute fast and easy start to purification work preprogrammed application protocols and method templates speed and reliability for every run fast optimisation and peak identificationaccurate result comparisons and peakfractionation column. Amersham biosciences akta prime fplc system manual. Akta laboratoryscale chromatography systems sevier lab. Feb 22, 2017 ge healthcare akta fplc frac950 fraction collector frac950 10593 duration.
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